However, approaches to cell culture are starting to change ... “Traditional tissue culture in 2D dishes and wells is pretty much obsolete at this point,” says Mann. “Those cells just ...

We routinely culture HESCs in p60 dishes. This protocol is based on culturing ... (iii) Gently detach cells with a cell scraper or a 10-ml pipet to collect most of the cells from the well or ...

The authors have generated important resources such as a reference dataset of early primate development by utilizing single-cell transcriptomic technology together with induced pluripotent stem cells ...

Cell culture is a fundamental technique in biotechnology and life sciences that involves growing and maintaining living cells outside their natural environment, typically in a controlled laboratory ...

and over time they will multiply to the point that we can expand the culture to more dishes, a process called "passaging." At left are images taken from two different embryonic stem cell (ESC ...