The study of the cell cycle focuses on mechanisms that regulate the timing and frequency of DNA duplication and cell division. As a biological concept, the cell cycle is defined as the period ...
When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...
Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...