3| Immediately transfer the calcium phosphate–DNA suspension into the medium above the cell ... assay for transient expression of the introduced DNA, harvest the cells 1–6 d after transfection.

50,000 or 100,000 cells per well without dramatic changes in transfection efficiency. Selecting the right cell density is assay dependent. For differentiation assays with Nanog-GFP cells ...

The CellTiter-Glo® Luminescent Cell Viability Assay uses luminescence to accurately determine the number of viable cells in a culture. Sequentially process plates exhibiting a highly consistent ...

Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.