Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization ... phosphate–DNA suspension into the medium above the cell monolayer. Use 0.1 ml of suspension for each 1 ...

Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers to deliver intracellular ... to discover an approach using the Neon NxT system that ...

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