Centrifuge the cells at 200g for 5 min at 15–25 °C and resuspend the pellet in 10 ml MEF medium supplemented with 4 ng/ml bFGF. To prepare the appropriate culture vessels for plating the ...

When scientists grow cells in culture, they fine tune factors such as medium ingredients, cell number, and incubator temperature to mimic the human body. However, one culture condition that ...

Next, the cell suspension was filtered through a 40-μm metal mesh once, and cells were disseminated into 250-mL (75 cm 2) gelatin-coated cell culture flasks (Falcon, Becton Dickinson, Heidelberg ...

1 Now, researchers can differentiate reprogrammed induced pluripotent stem cells (iPSCs) into any cell type and use them for numerous applications, including disease research, drug development, and ...

Stem cells produced in cell culture have the potential to be used to replace specialised cells in a person affected by disease or injury.