They explain how two opposing lenses, combined with the switching (on and off) of fluorescence between adjacent markers, can overcome the diffraction barrier. Although the examples given are ...

The waves always 'spread' to some extent into the area beyond the gap. This is diffraction - the spreading out of waves when they go through a gap, or past the edge of a barrier. The extent of the ...

Diffraction of light prevents optical microscopes from having spatial resolution beyond a value comparable to the wavelength of the probing light. This essentially means that visible light cannot ...