With this vector system, selection is more efficient, presumably because of the linked expression of both genes. Transfect the cells with the recombinant vector using Lipofectamine 2000 or another ...

Transform the verified clones into the cell lines of interest to test their ability to knock down gene expression. The vector(s ... products into PIR1-competent cells.

Expressing recombinant antibodies in mammalian cells is essential when post-translational ... The most common problems of antibody expression projects are inadequate antibody yield, lengthy ...

Shihabeddin et al utilized single-cell RNA-Seq analysis of adult P23H zebrafish animals to identify transcription factors (e2fs, Prdm1a, Sp1) expressed selectively in neural progenitors and immature ...