Fluorescence spectroscopy is a powerful, non-destructive technique widely used in biochemistry, biophysics, and structural biology. It is crucial for investigating enzymatic mechanisms and reaction ...
We conclusively demonstrate UV-fluorescence correlation spectroscopy (UV-FCS) on proteins with a single tryptophan residue. This unlocks the applicability of UV-FCS to a broad library of thousands of ...
The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP antisera. The GFP fluorescence (F) was quantified by fluorescence spectroscopy of whole-cell lysates.
fluorescent protein. Prior research has shown that synthesizing proteins can provide unique insights into the structure and function of natural proteins. To date, most such proteins are copies of ...