Here we describe optimal parameters for the multiphoton imaging and “greening” of ... normally quenched by FRET, is enhanced following selective bleaching of the red species by three-photon ...

The development and use of biosensors based on fluorescence resonance energy transfer (FRET) has allowed researchers to achieve real-time visualization of metabolite behavior or signal ...

FRET-FLIM optimization shows activity of two signaling molecules in single dendritic spine Max Planck Florida Institute for Neuroscience Journal Nature Methods Funder Rita Allen Foundation Scholar ...

FRET is a mechanism that describes how energy ... "These improvements to imaging and flow-based biomedical assays will enable the next generation of innovation," Squires said.