In particular, many have optimized their transfection reagents to deliver short pieces of RNA to the cytoplasm to silence gene expression (Box 3). Another focus for most companies is to develop ...

The major components of this technology are two-fold: the optimization of conditions for polyethylenimine (PEI)-mediated gene transfection in the bioreactor without the interference of ...

Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers to deliver intracellular molecules for cell engineering, gene expression studies ...

1 A control reporter driven by a strong, constitutive promoter is cotransfected with the experimental reporter plasmid to normalize for transfection efficiency and to account for the fact that ...