Sample quality directly influences the reliability of an experiment. Researchers executing high pressure liquid chromatography (HPLC) experiments must incorporate standardized weighing scales, ...

and electrical charge depending on the solvents used to carry the sample and the type of separator column. The Agilent 1200 Series HPLC quantifies separated molecules with an ultraviolet-visible ...

If you see bubbles in the syringe, empty the syringe into a waste beaker and redo Step #4. The sample will be injected to a port at the top of the HPLC. 6. Check that the knob, on the port at the top ...

High-speed counter current chromatography (HSCCC), an advanced liquid-liquid chromatography technique employing both a liquid ...

In this manner, protein loss owing to changes in mobile-phase pH is minimized on the RP-HPLC column. For sample collection from the preparative run, collect eluting protein peaks manually in ...

and electrical charge depending on the solvents used to carry the sample and the type of separator column. The current setup uses a size exclusion column column (SEC). After the molecules are ...

The contents of each flask were then diluted to volume with HPLC-grade water. A 5-mL portion of each diluted sample was further diluted to 50 mL with HPLC-grade water and 20 µg of internal ...