However, several histone modifications have similar DNA-binding patterns; an antibody that has not been rigorously tested against all histone PTMs might bind to the wrong type and deliver a false ...

Concentrations may vary among antibodies and should be determined ... using PCR primer pairs that amplify sequences for which the histone modification status is known. The purified DNA (we ...

and analyzed by immunoblotting with primary and secondary antibodies. Chemiluminescent signals were developed to identify histone modifications. Ribonucleic acid (RNA)-mediated interference ...

The Antibody-based Proteomics Core provides cutting edge antibody-based proteomic platforms for both validation and protein biomarker discovery, particularly for low abundance regulatory proteins and ...