Add 5 μl of the desired antibody to two of the three tubes ... using PCR primer pairs that amplify sequences for which the histone modification status is known. The purified DNA (we recommend ...

Figure 1: Time- and light-dependent phosphorylation of histone H3 in the suprachiasmatic ... overnight at 4°C with a rabbit polyclonal antibody (1:1000 final dilution, Upstate Biotechnology ...