We generated a new type of MHC multimers, which can be monomerized in the presence of a competitor, resulting in rapid loss of the staining reagent. Following dissociation, the T cells are ...

Add 25ml to each well of a microtiter plate or test tube. Prepare 2x staining cocktail containing all of your labeled reagents at the right titer. The suggested titer for the MHC tetramer reagent is 1 ...