We assessed six cell lines known to harbor Nras gene mutations using our multicolor multiplex assay. Two of the cell lines, MOLT-4 and PA-1, contain mutations in codon 12, while the remaining four ...

To help researchers configure single- or multiplex assays ... as 10 to 1 copies of target DNA and about 100–200 copies for RT-PCR,” says Shipley. “But the problem is sample purification ...

real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...