Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR ...

“Products created during setup decrease the efficiency of PCR,” says Joseph Donnenhoffer of Roche. The company's FastStart Taq polymerase has a one-base deletion that renders the enzyme ...

It degraded at 94°C, the temperature required to denature the DNA target; this meant that the scientists had to open the PCR reaction tube at the start of every cycle to add fresh enzyme. Additionally ...

Traditional microbiological tests, such as culturing bacteria on plates or using polymerase chain reaction (PCR) assays, often require specialized ... creating a measurable electrical output. The ...