“Products created during setup decrease the efficiency of PCR,” says Joseph Donnenhoffer of Roche. The company's FastStart Taq polymerase has a one-base deletion that renders the enzyme ...
A new technique, based on a chain reaction of recognition and hybridization events between two sets of DNA hairpin molecules, offers an enzyme-free ... chain reaction (PCR), for example ...
This method facilitates sequencing multiple regions of interest at the same time. Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, ...
Traditional microbiological tests, such as culturing bacteria on plates or using polymerase chain reaction (PCR) assays, often require specialized equipment and take ... creating a measurable ...
Since PCR's advent, scientists have developed filters to manage predictable stutter ... they analyzed amino acid sequences to identify mutations that enhanced the polymerase’s binding to its template ...