Many companies provide internet-based tools to help researchers design PCR primers and probes. But a new service provided by the National Cancer Institute will do the work for you. The ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

These tools are compatible with QIAcuity digital polymerase chain reaction (PCR ... the 'Design tool for Custom dPCR Microbial Assays', is a solution enabling the creation of tailored primers ...

allowing the design of appropriate primers and probes. qPCR shares the same limitations as PCR methods, including false-negative results due to inhibition of DNA polymerase or variations in the ...