Unlike beacons, Scorpions are PCR primer and probe in one. They consist of a primer that has a 5′ stem-loop tail with a fluorophore and a quencher in close proximity. This 'tail' will hybridize ...

Figure 1: Schematic diagram of the PCR–mediated recombination strategy used in constructing HIV–1 chimeric infectious clones. The 10–kb, PCR–amplified fragment was detected by gel ...