Unlike beacons, Scorpions are PCR primer and probe in one. They consist of a ... primers at two different concentrations—a limiting concentration and an excess one—so that when the limiting ...

The recommended final concentration is 1-10 percent. Formamide weakens base pairing and increases primer annealing specificity, thus enhancing PCR amplification for templates with high GC content.

USA). The concentration of the amino-modified oligonucleotides in PCR-water was 10 μM with 1% glycerol. Volumes of deposited probe solutions were about 250 pL, resulting in spots with a diameter ...