Moreover, antisense template generation by PCR requires the synthesis of quite long downstream primers, which is an expensive process when used for multiple probes. Certainly, innovative methods ...

But many researchers prefer to use this dye to optimize PCR and check that the primers are working well, before ordering a probe-based assay. A variety of probes specific for the amplified target ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Like Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling, enabling the option for a post-PCR melt curve analysis. These qualities make Scorpions ...

LGCs consultative approach to working with you, together with an industry-leading portfolio of probes and oligo modifications gives you confidence that you are getting the best PCR assay design to ...

qPCR, also known as real-time PCR ... designing these probes often requires extensive optimization. Appropriate controls in a qPCR assay ensure reliability and accuracy and help track any potential ...

These tools are compatible with QIAcuity digital polymerase chain reaction (PCR) systems and third ... enabling the creation of tailored primers and probes for bacterial, fungal, and viral targets.