“It's transfect with the ligase and peptide fusion constructs, add the dye and change the medium. There is basically no optimization,” says Ting. And PRIME is not plagued by the high ...

The current methods also incur high costs, and are difficult to scale up, resulting in limited and expensive supplies of important peptide medicines. The team in Manchester searched for new ligase ...

The most expensive reagents are the biotin ligase (BirA from Avidity, LLC) and the PE-conjugated streptavidin (Prozyme ... The stability of MHC class I tetramers is variable and is influenced by the ...