Out-of-focus light is the reason why wide-field microscopy is usually limited to the study of thin specimens. The key part of a confocal microscope is the pinhole placed in front of the detector at ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Because CNS tissue is very dense and scatters light, fluorescently dyed brain cells looked blurry when viewed under a conventional widefield microscope. To counter this problem, Minsky placed a ...

By combining the pinhole-plane imaging concept of Airyscan 2 with improved illumination and detector readout routines, the new Multiplex mode extracts more spatial information, enabling parallel ...

The microscope stage is based on an OpenFlexure "Delta" stage, and the confocal optics are constructed with a 3D printed body, some lenses, a 405nm laser, pinhole and photodiode. The microscope is ...

There are dual Nipkow disks with 50 or 25-micrometer lens pinhole arrays. The stage is motorized in X ... Three environmentally controlled chambers include: 1) 1x35mm dish, 2) 3x35mm dishes and 3) low ...