In principle, cloning PCR products necessitates some ... insertions or deletions in plasmid DNA. “The use of Phusion Hot Start DNA polymerase in amplification guarantees extreme fidelity and ...
In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...
In this GEN webinar, Elegen’s Galit Meshulam-Simon, PhD, and Matt Deaner, PhD, will discuss recent innovations in DNA ... free cloning technology for highly accurate fragments for plasmid ...
To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...
a cost-effective method to generate recombinant rabbit monoclonal antibodies and a platform for the combination of multiple DNA constructs into a single plasmid vector with the unique feature of ...
Service can be initiated at the cloning stage where we clone your transgene into the adenovirus backbone plasmid, at the transfection stage ... Several plaques are selected, expanded, HIRT DNA is ...
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