Less than 1 percent of the initial cells fuse to form hybridoma cells. Unused B cells in the culture stop dividing naturally, while chemotherapy destroys the unfused myeloma cells. Researchers use HAT ...

Existing processes used for developing stable cell lines for the production of biotherapeutics ... whether individuals are screening CHO cells, hybridoma populations, whole B cell repertoires ...

Before adding cells, supplement the medium with any ... single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies. J. Immunol. Methods 50, 161 ...

Antibodies bind to specific antigens on pathogens. This means that only one type of antibody will bind to a matching antigen. For example, an antibody that can recognise an antigen on the ...