Wash the resin in a disposable standing column with 2 ml of wash buffer and then elute His-tagged protein with wash ... phenol/chloroform/isoamyl alcohol extraction. We recommend using pMRBAD ...

Image Credit: Norgen Biotek Corporation Figure 2. High-Quality Total Proteins Eluted in Mass Spec-Compatible Buffer. Norgen's RNA/Protein Purification Plus Kit provides a column purification step ...