GST lysis buffer consists of 20 mM Tris-Cl (pH 8.0 ... 8| Optional: Elute the GST fusion protein and any proteins bound to it by adding 50 μl of 20 mM reduced glutathione in 50 mM Tris-Cl ...

Pellet cells at 300g at 4 °C for 10 min in a centrifuge. 3| Resuspend the cell pellet in 5 ml of ice-cold NP-40 lysis buffer. Incubate on ice for 5 min. Pellet the nuclei at 120g at 4 °C for 10 ...