The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the forward primer in a final 50 μl reaction volume (Perkin-Elmer Standard PCR reaction buffer, 0.2 mM dNTPs ...

Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme ... Formamide weakens base pairing and increases primer annealing specificity, thus ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...