MS, an experimental protocol combining high-resolution protein fractionation using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with the latest mass spectrometry technology to enable highly ...
After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here 1 uses ...
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