Precipitates were washed four times with IPH buffer (1 ml), separated on 10% SDS–PAGE gel and blotted onto ... Immunoprecipitation of histone deacetylase activity from cell extracts.

High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100 ... using PCR primer pairs that amplify sequences for which the histone modification status is known. The purified DNA (we recommend ...