before ordering a probe-based assay. A variety of probes specific for the amplified target (or amplicon) can be used in real-time PCR. By far the favorite, especially among scientists who have ...

The specificity of ProbeLibrary assays was analyzed by real-time PCR amplification of different templates containing 0, 1 or 2 mismatches relative to the ProbeLibrary probe (Fig. 3c). Fluorescence ...