In the single-tube operation ... by hybridizing a primer to the beads that is complementary to Tag1. The signal obtained represents the maximal signal possible, as every PCR product on the bead ...

I therefore devised a single-tube, nested PCR method that is easy to handle and yields consistent results. The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

1 Denaturation: Double-stranded DNA separates into single strands. Annealing: Short DNA fragments bind complementary ... Formamide weakens base pairing and increases primer annealing specificity, thus ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...