We tested this system using 30-ml cultures in shake flasks and were able to scale up the production process using the same transfection procedure irrespective of the final culture volume (5-liter ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

The lack of lipid-based transfection products that efficiently transfect these cell types has challenged researchers for many years. To overcome these obstacles, Lipofectamine 3000 is designed to ...