1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells 1,2. Electroporation works well with cell lines that are refractive to other techniques, such as calcium ...

Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers to deliver intracellular molecules for cell engineering, gene expression studies, ...