该研究通过构建阿拉伯糖诱导的Tn5转座酶系统,实现了细菌突变群体的时空可控性,成功在《Nature Microbiology》发表了题为"Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice"的重要研究成果。 研究团队采用了几个关键技术方法:1 ...
Recently, transposon-mediated insertional mutagenesis has become available and large-scale insertional mutagenesis projects with piggyBac 40 or sleeping beauty 41 transposons have been proposed.