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噬菌体携带孤儿抗毒素样酶来中和DarTG1毒素-抗毒素防御系统
纯化后的蛋白质在液氮中速冻,储存在 - 80°C。 ADP - 核糖糖水解酶试验:将含有 pJB37 - darT1 的大肠杆菌 dh5a 过夜培养物在含有 0.4% 葡萄糖的培养基中培养,然后以 1:100 的比例稀释到含有葡萄糖的新鲜培养基中,培养至 OD600 约为 0.3。同时,将培养物进行系列稀释 ...
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