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生物通
21 天
《Nature Methods》新型 FRET 生物传感器实现 PTEN 活性在体内的高分辨 ...
这样,当 PTEN 构象发生变化时,就会引起 FRET 效率改变,通过 2pFLIM 测量 GFP 的荧光寿命,就能反映 PTEN 的活性。为了找到最佳的传感器设计,他们对连接区域进行了优化。实验发现,优化后的传感器在基础状态下有较高的 FRET 水平(低荧光寿命),而当 PTEN 的四 ...
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