GAS2 is a cytoskeletal regulator that interacts with both F-actin and microtubules. Here, cryo-EM structures of GAS2 domains ...
接着,作者使用GFP-RFP-LC3慢病毒感染THP-1细胞 ... 当P. g.刺激8h时,自噬小体的数量增加,而自噬溶酶体的数量在刺激组中相比对照组减少(该结果与WB结果指标变化一致),说明P. g.刺激抑制自噬溶酶体的生成。为了进一步证明此结论,作者使用雷帕霉素激活 ...
2.3 Construction of mouse IFN-γ and sPD-1 viral plasmids PCR primers were designed to include the target genes for mouse IFN-γ and sPD-1 with BP recombination, and the IRES-GFP (green fluorescent ...
The study by Pudlowski et al. shows that a previously-identified protein complex, composed of delta- and epsilon-tubulin together with TEDC1 and TEDC2, functions in generating centriolar triplet ...