An in vivo glioma model was generated by implanting GOLPH3-knockdown U87 cells into nude mice. Apoptosis was assessed using flow cytometry, immunofluorescence staining, TUNEL assays, and Western ...

Feb. 4, 2025 — Research has shown that a potential new targeted therapy for childhood brain cancer is effective in infiltrating and killing tumor cells in preclinical models tested in mice.

Feb. 12, 2025 — Scientists have uncovered the equipment that enables bacteria to survive exposure to antibiotics: a shuttling mechanism that helps a complex of proteins pump out a wide spectrum ...

for their assistance with aortic sinus immunofluorescence microscopy. They thank the University of Ottawa Heart Institute High Resolution Cell Imaging Core Facility for their help with aortic sinus ...

Tissues or cells were lysed using lysis buffer (50 mM tris (pH 7.5), 150 mM NaCl ... staining or 4% PFA fixative solution for immunofluorescence or TUNEL assay. The pictures were captured using a ...