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海马CA2神经元中MCU的表达调节树突线粒体形态和突触可塑性
通过对不同脑区的荧光强度进行量化,验证 MCU 在 CA2 神经元中的敲除效果,以及检测 cFOS 阳性细胞的表达情况。 蛋白质保留扩展显微镜技术(ProExM):对 40μm 的小鼠脑切片进行免疫染色,利用 Amigo2 - EGFP 标记 CA2 区域,随后进行 4× 蛋白质扩展显微镜处理 ...
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