The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

Herein, we updated our 2018 protocol 1 by implementing a sonication step into urea-based tissue lysis to improve the detection of membrane-bound and DNA-binding proteins.

Efficient extraction of membrane proteins can be a dicey business: Add too little detergent, and you fail to extract the protein; add too much, and purification procedures and downstream applications ...