Both letters question the association between E1A and EWS–FLI1 by studying HEK293 cells using RT–PCR. HEK293 cells derive from human embryonic kidney cells immortalized by adenoviruses many ...

Mito-Tracker Green 是一种绿色荧光染料，生产厂商是西安强化生物科技。可以选择性地积聚在线粒体基质中。 MitoTracker Green FM 通过与半胱氨酸残基的游离硫醇基反应共价结合线粒体蛋白，从而可以不依赖膜电位即对线粒体膜电位进行染色。 MitoTracker Green的工作原理 ...

1. 在HeLa细胞中，探针与MitoTracker Green共定位系数达0.91，表明特异性线粒体靶向。 Cyanine5.5-PCA实现线粒体氧化还原状态的动态监测，为线粒体相关疾病的治疗提供定量评估手段，并提示PCA作为线粒体靶向药物前体的潜力。 CY5聚乙二醇羧基,CY5-PEG羧基,CY5-PEG-COOH MW2000 ...

For mitochondrial staining, cells were incubated in full media at 37°C for 30 min in the presence of 100 nM Mitotracker Deep Red FM and 100 nM Mitotracker Green (both Invitrogen, UK). Cells were ...

In an experiment using DMEM incubated for 24 h, HG intervention notably elevated PINK1 and LC3B expression. In addition, HG exposure reduced the levels of cytochrome c oxidase subunit 4 and decreased ...

CAR-T cells alone or co-cultured with SEM at a 1:1 ratio for the indicated time, and 200 nM TMRE (MedChemExpress, HY-D0985A) was used to detect CAR-T cells mitochondrial activity, 200 nM MitoTracker ...

The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville 3010, Australia ...

同时，线粒体膜电位下降，mtROS 水平显著升高。 通过共转染 GFP-LC3、LysoTracker 和 MitoTracker 评估自噬活性，发现感染 24 和 48 小时后，GFP-LC3 和 LysoTracker 与 MitoTracker 的共定位显著增强。此外，自噬相关蛋白 Parkin、PINK1 和 LC3-II 的表达随时间显著上调，p62 表达下调。

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Glioblastoma multiforme (GBM) is the most ...

For decades, animal models have been the gold standard for preclinical testing of potential drugs. Yet, even the best animal models are not perfect. For example, small rodent species with ...

Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...