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寡核苷酸退火实验方案 | 默克生命科学
本实验方案用于两条序列互补的单链寡核苷酸的退火反应(图1)。加热后冷却有助于杂交反应的进行。 图 1.退火反应示例图 图 1.退火反应示例图。加热“打破”了所有的氢键,从而破坏了每个寡核苷酸内的全部二级结构。随后,缓慢的冷却促进了杂交反应 ...
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