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解析拟南芥 GORK 通道结构与功能,解锁气孔工程新靶点
通过定点突变和基因编辑技术构建多种 GORK 突变体,并将其在非洲爪蟾卵母细胞(Xenopus laevis oocytes)中表达,利用双电极电压钳技术记录 通道电流,分析突变对通道功能的影响。 尽管尚未解析出 GORK 真正的开放状态结构,但研究确定了围绕 C - 连接子、CNBHD 和 ...
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