But many researchers prefer to use this dye to optimize PCR and check that the primers are working well, before ordering a probe-based assay. A variety of probes specific for the amplified target ...

In this study, three pairs of specific primers and probes targeting the ASFV B646L gene, F1055L gene, and E183L gene were designed to detect universal, genotype I, and genotype II strains, ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. Leveraging this information, we developed a TaqMan probe-based quantitative real-time PCR (qPCR) assay ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

7 18 19 In the current study, we were able to rapidly screen for the detection of the virus genome of all eight types of human herpes viruses by using several different primer pairs. When positive ...

Design degenerated primers on highly variable alignments for full genome sequencing or qPCR. Specifically developed for viruses.

A second primer binds to the transcript and ... the HBV DNA in the sample is amplified in a non-nested PCR in the presence of specific TaqMan probes or fluorescent dyes in order to monitor the ...