But many researchers prefer to use this dye to optimize PCR and check that the primers are working well, before ordering a probe-based assay. A variety of probes specific for the amplified target ...

all the required probes for analysis of virtually any gene are easily stored in a standard laboratory freezer. Consequently, probe synthesis and delivery delays are eliminated. The PCR primers ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

A Chinese research team has successfully developed a triplex real-time quantitative fluorescence PCR method capable of ...