Moreover, antisense template generation by PCR requires the synthesis of quite long downstream primers, which is an expensive process when used for multiple probes. Certainly, innovative methods ...

But many researchers prefer to use this dye to optimize PCR and check that the primers are working well, before ordering a probe-based assay. A variety of probes specific for the amplified target ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Like Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling, enabling the option for a post-PCR melt curve analysis. These qualities make Scorpions ...

qPCR, also known as real-time PCR ... designing these probes often requires extensive optimization. Appropriate controls in a qPCR assay ensure reliability and accuracy and help track any potential ...

Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today released the One-Step RT-ddPCR Advanced Kit for Probes which combines reverse transcription and Droplet Digital PCR (RT-ddPCR) for absolute RNA ...