But many researchers prefer to use this dye to optimize PCR and check that the primers are working well, before ordering a probe-based assay. A variety of probes specific for the amplified target ...

all the required probes for analysis of virtually any gene are easily stored in a standard laboratory freezer. Consequently, probe synthesis and delivery delays are eliminated. The PCR primers ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

A Chinese research team has successfully developed a triplex real-time quantitative fluorescence PCR method capable of ...

The RVP employs a multiplex PCR with labelled primers and a single-step hybridization ... of 5 × FastStart DNA Master Plus Hybridization Probes (Roche Diagnostics, Mannheim, Germany), 200 nM ...

BHQ TM Probes are traditional ... Thanks to the large array of dyes they offer, you can develop the ideal PCR or qPCR probe for your application without losing performance. Each dye is carefully ...