The maternal rats were anesthetized with isoflurane and kept anesthetized during milk extraction ... 1% Triton-X100, 0.5% sodium deoxycholate, 1 mM PMSF, and 10 μg/ml leupeptin), incubated on ice for ...

Membrane proteins are proteins found in cell membranes, either at the surface or on intracellular organelles. Integral membrane proteins such as receptors and ion channels span the membrane.

Protein design (or protein engineering) is a technique by which proteins with enhanced or novel functional properties are created. Proteins can be engineered by rational design, which typically ...

Scientists adopted similar approaches to utilize a new generation of engineered antifreeze and ice-binding proteins as bio cryoprotective agents for preservation and industrial applications. There are ...

10 mM sodium azide in DPBS). Cells were washed once with PBS, and collected by trypsinization, and pelleted at 500 x g. SILAC lysate preparation: Protein concentration was measured using Quibit ...

This depends on the type of enzyme. The enzyme pepsin breaks down proteins in the acidic conditions of the stomach. Pepsin has an optimum of pH 2.5 and a working range of between pH 1-4.