8| Add 1 μl of a dNTP solution (each at 10 mM) and 2 μl (5 units) of bacteriophage T4 DNA polymerase. Incubate the reaction mixture at 15–25 °C for 15 min. Set aside a small aliquot (3 μl ...

The high accuracy or fidelity of semi-conservative DNA replication is the product of nucleotide template interaction, polymerase-enhanced base selectivity, exonuclease proofreading and the post ...

E.coli XL-1Blue was obtained from Agilent Technologies(USA). M13KO7 helper phage was obtained from NEB(Beijing, China). PrimeSTAR ® HS DNA Polymerase was from Takara(Beijing, China). T4 DNA ligase and ...

Results: These findings revealed that TAEs can be quantitatively detected using DNA-mediated quantitative polymerase chain reaction (qPCR ... The SA mixture, proximity cnct, T4 DNA Ligase, and T4 DNA ...

Learning about your genetics and ancestry is easier than ever. All you need to do is choose from the many DNA ancestry tests available online, receive your kit and send back the required DNA samples.

Now, researchers used cryoelectron microscopy (cryo-EM) to elucidate the structure of the Nipah virus polymerase complex (the large protein and phosphoprotein). Not only that, but they also ...

Comparison of that ancient DNA with modern human DNA showed that the two species had interbred and that people today still carry the genetic fingerprint of that intermixing. Since then ...

While most known types of DNA damage are fixed by our cells' in-house DNA repair mechanisms, some forms of DNA damage evade repair and can persist for many years, new research shows.

Of course, though, testing a child's parentage based on physical features is far too subjective — that's typically where DNA tests come in. Such tests can offer a far more scientific way of ...

During the peer-review process the editor and reviewers write an eLife assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) ...

Genomic DNA was isolated from either embryos or fin clips using HotSHOT method (Meeker et al., 2007). To identify genomic lesions, the following primers were used: aqp1a1-fwd ...

The number of home DNA test kits offering nutrition and diet advice matched to your genes is growing. They’re based on the idea that each of us responds to foods differently, in part due to our ...