Protein lysate was prepared as we previously described (40, 41). Tissues or cells were lysed using lysis buffer (50 mM tris (pH 7.5), 150 mM NaCl, 5 mMEDTA, and 0.1% NP-40). For human sperm cells, ...

RNA extraction from exosomes and quality control RNA lysis buffer was added to the thawed exosome extract ... Total RNA was isolated from the samples using TRIzol reagent (Invitrogen, United States) ...

One of the most picturesque spots on Capri, Villa Lysis offers a bird's-eye view of the towns of Capri and Anacapri, as well as Marina Grande. The expansive grounds of this 20th-century villa ...

This important paper describes the regulatory pathway of rRNA synthesis by Meioc-Piwil1 in germ cell differentiation in zebrafish. Using the molecular genetic and cytological approaches, the authors ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Lipid transfer proteins mediate the ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Corneal diseases can cause severe visual ...

Following a 48-hour infection period, puromycin (2 μg·mL-¹) was used to select for stable knockdown/overexpression cell lines. Total RNA was extracted from cells using Trizol (Takara, Dalian, China) ...

The David Rockefeller Studies Program—CFR's think tank—analyzes pressing global challenges and offers actionable steps that policymakers and citizens can take to address them. The more than ...

TRIzol reagent (15596018), JC1 dye (T3168), Mitosox red (M36008), Alexa Fluor (A11008), Alexa Fluor (A11001), and mounting medium (P36931) were all acquired from Fisher Scientific, Invitrogen ...

This can lead to a potentially severe condition called tumor lysis syndrome (TCS), characterized by nausea, vomiting, muscle cramps, fatigue, irregular heart rate, restlessness, confusion, and seizure ...